The Knight ADRC has supported many investigators at Washington University and at other institutions over the years. We wish to avoid the situation where two investigators study the same research question to avoid duplication of effort and potential conflict. To determine if your topic has already been studied with our resources, please search our database. If you find that your topic or a related topic has been submitted, you may wish to contact the investigator to inquire about their findings to determine how you might proceed. You may wish to collaborate or modify your request to avoid overlap. The results below reflect requests made since online requests have been accepted. As such, not all fields will have data as certain information, such as aims, were not collected until recently. If an entry has been assigned an ID number (e.g. T1004), the full request has been submitted and is either approved, disapproved or in process. If an entry has no ID number, then it represents a submission that has not yet been reviewed. Search terms are applied across an entire requests application including variables not displayed below. A more specific, detailed search may yield better results depending upon your needs.
Investigator: Hongyu An
Project Title: Neuroinflammation in Cerebral Small Vessel Disease using PET/MR Imaging
Date: March 14, 2023
Request ID: T2302
Aim 1: Determine if S1PR1 expression increases and colocalizes with microglia and astrocytes in post-mortem brain specimens from deceased patients with underlying CSVD.
Aim 2: Determine if neuroinflammation measured by 11C-CS1P1 uptake is independently associated with CSVD structural endpoints using in vivo PET/MR imaging.
Investigator: Zachary McEachin
Project Title: Assessment of cryptic exons in TDP-43 proteinopathies
Date: February 14, 2023
Request ID: T2301
Aim 1: Determine the abundance of cryptic exons across FTLD-TDP subtypes
Aim 2: Determine the proteomic signatures associated with distinct TDP-43 subtypes
Project Title: Tissue request T1225. Second Amendment Brain RNA levels, protein levels and Alzheimer’s Disease: deep molecular profiling
Date: February 12, 2023
Request ID: T1225-B
Aim 1: Identification and Characterization of Cell-Specific Transposable Elements Implicated on Alzheimer Disease and Healthy Aging
Aim 2: To generate neuron and microglia specific ATAC-seq, CAGE-seq, DNA methylation and long-reads RNA-seq
Investigator: A/Prof Matthew Pase
Project Title: Plasma inflammatory biomarkers for the diagnosis of dementia subtypes
Date: February 8, 2023
Aim 1: To determine the diagnostic accuracy of selected blood neuroinflammatory biomarkers
Aim 2: To determine if blood biomarkers of neuroinflammation correlate with established neurodegenerative disease biomarkers
Investigator: Chengjie Xiong
Project Title: R01 Cross Sectional and Longitudinal Racial Disparity in Molecular Biomarkers of AD
Date: November 25, 2022
Request ID: T2219
Aim 1: To implement a central and standardized processing protocol for retrospectively and prospectively collected plasma samples and a harmonized assessment protocol of SDOH across the sites, and to determine cross-sectional and longitudinal racial differences in plasma amyloid and pTau biomarkers.
Aim 2: To determine racial differences in the correlations of plasma biomarkers with CSF and imaging biomarkers, as well as concurrent and future clinical and cognitive outcomes.
Aim 3: To assess racial differences in plasma NfL and GFAP, and whether the differences are modified by risk factors for vascular disease: MRI white matter hyperintensities (WMH), BMI, and HbA1c.
Investigator: Marta Barrachina
Project Title: Identification and characterization of mitochondrial methylcytosines as novel blood-based biomarkers for predicting Alzheimer’s disease dementia progression at mild cognitive impairment stage
Date: November 17, 2022
Request ID: T2218
Aim 1: To develop a model to classify patients diagnosed with Mild Cognitive Impairment susceptible to progressing to Alzheimer’s disease dementia.
Investigator: Bruno A. Benitez and Collaborators: Oscar Harari and Mark Sands
Project Title: Multi-omic approach to Autosomal dominant adult-onset neuronal ceroid lipofuscinosis
Date: November 15, 2022
Request ID: T1108-B
Aim 1: Aim 1: To establish a cellular and molecular map of DNAJC5-associated neurodegenerative pathways.
Investigator: Carlos Cruchaga
Project Title: Functional characterization of novel Alzheimer disease related genes using induced pluripotent stem cell models
Date: October 14, 2022
Request ID: T2217
Aim 1: To determine whether novel variants on chromosomes 3 and 19 modify sTREM2 levels and impact TREM2 and microglia function in cellular models
Aim 2: To study the effect of Chr 3 gene region OSTN/GMNC/IL1RAP on tau and calcineurin metabolism in iPSC-derived neurons
Investigator: Suzanne Schindler
Project Title: Evaluation of plasma tau in the Knight ADRC cohort
Date: August 21, 2022
Aim 1: Measurement of plasma p-tau in most Knight ADRC samples
Aim 2: Extensive characterization of plasma tau species in a subset of informative samples
Investigator: Diana Farmer/Aijun Wang (mPI)
Project Title: Engineered Targeted Extracellular Vesicles for Neurovascular Unit Restoration in Brain Organoid Model of Alzheimer’s Disease
Date: June 19, 2022
Request ID: T2215
Aim 1: Generate Ex Vivo Model of Alzheimer’s disease Using Human Brain Organoids Derived from Induced Pluripotent Stem Cells
Aim 2: Examine preferential neuron uptake, reduced blood-brain barrier permeability, increased angiogenesis, and glial cell modulation by astrocyte-derived extracellular vesicles in human brain organoid model