The Knight ADRC has supported many investigators at Washington University and at other institutions over the years. We wish to avoid the situation where two investigators study the same research question to avoid duplication of effort and potential conflict. To determine if your topic has already been studied with our resources, please search our database. If you find that your topic or a related topic has been submitted, you may wish to contact the investigator to inquire about their findings to determine how you might proceed. You may wish to collaborate or modify your request to avoid overlap. The results below reflect requests made since online requests have been accepted. As such, not all fields will have data as certain information, such as aims, were not collected until recently. If an entry has been assigned an ID number (e.g. T1004), the full request has been submitted and is either approved, disapproved or in process. If an entry has no ID number, then it represents a submission that has not yet been reviewed. Search terms are applied across an entire requests application including variables not displayed below. A more specific, detailed search may yield better results depending upon your needs.
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Investigator: Nicholas M. Kanaan
Project Title: Development of Novel Tau SIMOA Assays
Date: [208]
Request ID: T2129
Aim 1: Develop tau assays that detect total tau by targeting specific epitopes throughout the tau protein.
Aim 2: Develop tau assays that detect specific pathogenic forms of tau that are associated with human diseases.
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Investigator: Takahisa Kanekiyo/Anne M. Fagan
Project Title: Biology and pathobiology of apoE in aging and Alzheimer’s disease/Biomarker Core
Date: [208]
Request ID: T2128
Aim 1: Establish apoE-related fluid biomarkers for clinical dementia progression
Aim 2: Uncover potential fluid biomarkers for clinical dementia progression
Aim 3: Generate a fluid biomarker dataset to elucidate the effects of APOE genotype on clinical dementia progression
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Investigator: Timothy Miller
Project Title: Generation of a PET Tracer for TDP-43 Aggregates for ALS and FTD
Date: [208]
Request ID: T2127
Aim 1: Develop bioassays to assess binding affinity to aggregated TDP-43
Aim 2: Identify lead compounds with high binding affinity to aggregated TDP-43
Aim 3: Synthesis of TDP-43 targeted PET radiopharmaceuticals and assessment of radiochemistry, BBB permeability, and binding affinity to aggregated TDP-43
Aim 4: Optimization of lead TDP-43 PET radiopharmaceuticals via pharmacokinetic studies in rodent models

Investigator: Gregory J Zipfel
Project Title: Impact and Mechanisms of Vascular Oxidative Stress on CAA Pathogenesis and CAA-related Dementia
Date: [208]
Request ID: T1919-A
Aim 1: Correlate HSPG expression with NADPH oxidase activity, vascular oxidative stress, altered LRP1 and oxidized-LRP1 levels, and CAA in cerebral vessels of Alzheimer�s and non-Alzheimer�s autopsy samples
Aim 2: Correlate NADPH oxidase activity with vascular oxidative stress, altered LRP1 and oxidized-LRP1 expression, and CAA load in cerebral vessels of Alzheimer�s and non-Alzheimer�s autopsy samples
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Investigator: Yuchao Chen
Project Title: Multi-omics Profiling of Extracellular Vesicles for Diagnosis of Alzheimer’s Disease
Date: [208]
Request ID: T2125
Aim 1: Isolation of extracellular vesicles from plasma with high purity and high yield.
Aim 2: Transcriptome and proteome profiling of purified EVs.
Aim 3: Identify and validate an extracellular vesicle-based biomarker panel for Alzheimer’s disease
Aim 4: Establish a prediction model for Alzheimer’s disease

Investigator: Janardan P. Pandey
Project Title: Immunoglobulin GM Allotypes in Alzheimer’s Disease
Date: [208]
Request ID: T2124
Aim 1: Determine if GM allotypes are risk factors for Alzheimer’s disease in African Americans
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Investigator: Judith Steen
Project Title: Comparison of P301S and P301L mouse models with Human subjects
Date: [208]
Request ID: T2123
Aim 1: Profile P301S human patient tissue with FLEXITau
Aim 2: Profile P301S human patient tissue with global proteomics
Aim 3: Compare the human and mouse model Tau PTM profiles
Aim 4: Compare global proteomics data from human and mouse models with the P301S mutation

Investigator: Einar Sigurdsson
Project Title: Cell-Type Specific Vulnerability to Tauopathy and Its Prevention in Multiple Models
Date: [208]
Request ID: T2122
Aim 1: To clarify cell-type specific vulnerability in Drosophila melanogaster tauopathy models.
Aim 2: To clarify in the fly tauopathy models the mechanisms of sdAb- mediated clearance of tau pathology, associated prevention of neurotoxicity, and tau-induced death.
Aim 3: Confirmation of key findings from Aims 1-2 in tauopathy mouse models and in human tauopathy neurons derived from induced pluripotent stem cells (iPSCs) and fibroblasts.
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Investigator: Luca Giliberto
Project Title:
Date: [208]
Request ID: T2121
Aim 1: 1. Determine whether loss of Irf5 enhances the susceptibility of mice to pathogenic tau accumulation and pathology in the brain.
Aim 2: 2. Determine whether therapeutic inhibition of IRF5 protects P301S mice from disease onset, progression and neurodegeneration.
Aim 3: 3. Determine the mechanisms by which IRF5 participates in immunosurveillance of pathological tau. Transcriptional profile of tau-driven, IRF5-dependent microglia activation.
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Investigator: Nicolas Barthelemy
Project Title: CSF MTBR-tau243: a fluid biomarker to track tau pathology across the AD continuum
Date: [208]
Request ID: T2120
Aim 1: Validation of novel AD fluid biomarkers to track tau tangles in brain
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