The Knight ADRC has supported many investigators at Washington University and at other institutions over the years. We wish to avoid the situation where two investigators study the same research question to avoid duplication of effort and potential conflict. To determine if your topic has already been studied with our resources, please search our database. If you find that your topic or a related topic has been submitted, you may wish to contact the investigator to inquire about their findings to determine how you might proceed. You may wish to collaborate or modify your request to avoid overlap. The results below reflect requests made since online requests have been accepted. As such, not all fields will have data as certain information, such as aims, were not collected until recently. If an entry has been assigned an ID number (e.g. T1004), the full request has been submitted and is either approved, disapproved or in process. If an entry has no ID number, then it represents a submission that has not yet been reviewed. Search terms are applied across an entire requests application including variables not displayed below. A more specific, detailed search may yield better results depending upon your needs.
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Investigator: Carlos Cruchaga
Project Title: Brain samples from the Neuropathological core to Cruchaga lab and Genetics and High Throughput -Omics Core
Date: October 23, 2025 at 9:56 am
Request ID: T2519
Aim 1: Aim 1. To collect participant biological materials, obtain and bank DNA and generated genetic information.
Aim 2: Aim 2: To extract RNA from all the brain samples in the Neuropath core
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Investigator: Li-Huei Tsai
Project Title: Investigating Lipidomic Perturbations in the CSF with Age and Alzheimer’s Disease Progression: Toward Mechanistic Insights and Accessible Lipid Biomarkers
Date: October 15, 2025 at 3:41 pm
Request ID: T2518
Aim 1: Perform lipidomic profiling on longitudinal CSF samples from 75 ADRC Knight subjects using LC-MS based methods to identify lipid changes that occur with age and their association with CSF Abeta42/40 levels, sex, and APOE4 carrier status.
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Investigator: Carlos Cruchaag
Project Title: Longitudinal plasma proteomic studies in Alzheimer’s Disease
Date: October 15, 2025 at 11:28 am
Request ID: T2517
Aim 1: Identify proteomic profiles and biomarkers to progression to symptomatic AD and MCI-to-AD
Aim 2: Identify proteomic profiles associated with the rate of progression. i.e: fast vs slow progressors
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Investigator: Jessica Mozersky & Sarah Hartz
Project Title: Comparative plasma validation
Date: September 17, 2025 at 3:57 pm
Request ID: T2515
Aim 1: Compare & map p-tau 217% measures from the Bateman lab to those from C2N
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Investigator: Carlos Cruchaga
Project Title: Large Scale Phosphoproteomics to identify novel biomarkers and causal targets
Date: September 14, 2025 at 11:24 am
Request ID: T2516
Aim 1: Identify plasma proteomic signatures and new prediction models for AD.
Aim 2: AI-driven multi-disease classifier
Aim 3: Identify potential causal and druggable proteins by using pQTL and Mendelian randomization, colocalization, and PWAS approaches.
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Investigator: Maria Catarina Silva
Project Title: Defining 4R-Tau Pathology in a New Cohort of IVS10+16 Neuronal Models for Therapeutic Development
Date: September 10, 2025 at 4:00 pm
Request ID: T2514
Aim 1: Model IVS10+16 tauopathy in iPSC-derived neurons.
Aim 2: Model IVS10+16 tauopathy using age-relevant neurons generated by direct transdifferentiation (MiN).
Aim 3: Dual neuronal cell models to support and evaluate novel small molecules therapeutics potential.
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Investigator: Andrew Yoo
Project Title: Modeling Neuronal Aging and Alzheimer’s Disease via 3D Direct Reprograming of Patient Fibroblast
Date: September 8, 2025 at 11:45 am
Request ID: T2508
Aim 1: Epigenetic basis for retrotransposon element dysregulation in neuronal aging
Aim 2: Effect of reducing RCAN1 function on neurodegeneration of directly reprogrammed AD neurons
Aim 3: Establishment of isogenic neuronal aging models through direct reprogramming
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Investigator: Miranda Orr
Project Title: Impact of Fixation and Post Mortem Interval Variables on Spatial Omics Data
Date: August 29, 2025 at 4:38 pm
Request ID: T2513
Aim 1: Test how ADRC fixation variables impact spatial proteomics data
Aim 2: Test how post mortem interval impact spatial proteomics data
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Investigator: Elizabeth Pollina
Project Title: Activity-dependent genome plasticity in human aging and disease
Date: August 28, 2025 at 3:34 pm
Request ID: T2512
Aim 1: Characterize activity-dependent transcription and DNA damage repair in young, old and LOAD neurons.
Aim 2: Assess effects of NPAS4:NuA4 complex manipulation on ameliorating AD phenotypes.
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Investigator: Justin Melendez
Project Title: Pseudotime Analysis of CSF Proteomics and Lipidomics for Alzheimer’s Disease Biomarker Discovery and Pathway Elucidation, Validated by Longitudinal Data
Date: August 15, 2025 at 11:10 am
Request ID: T2511
Aim 1: Develop and validate a pseudotime analysis of CSF proteomics to identify protein changes linked to amyloidosis progression and aging. Validate the model using longitudinal CSF data to pinpoint early biomarkers and drivers of amyloidosis.
Aim 2: Investigate the pseudotime trajectories of CSF proteomic changes in APOE4 carriers versus non-carriers to identify distinct aging and amyloidosis pathways. Validate these differences using longitudinal CSF data to identify specific biomarkers and pathways linked to APOE4-related Alzheimer’s risk.
Aim 3: Perform lipidomic profiling on longitudinal CSF samples using LC-MS to identify lipid biomarkers predictive of Alzheimer’s risk. Examine the influence of age, sex, APOE4 status, and Alzheimer’s pathology, and integrate proteomic and lipidomic data for comprehensive biomarker development.
Aim 4: Integrate lipidomic data with pseudotime analyses to explore combined proteomic and lipidomic changes over time. Use longitudinal CSF data to validate the integrated model, aiming to uncover comprehensive biomarkers and pathways that predict Alzheimer’s risk and progression.