The Knight ADRC has supported many investigators at Washington University and at other institutions over the years. We wish to avoid the situation where two investigators study the same research question to avoid duplication of effort and potential conflict. To determine if your topic has already been studied with our resources, please search our database. If you find that your topic or a related topic has been submitted, you may wish to contact the investigator to inquire about their findings to determine how you might proceed. You may wish to collaborate or modify your request to avoid overlap. The results below reflect requests made since online requests have been accepted. As such, not all fields will have data as certain information, such as aims, were not collected until recently. If an entry has been assigned an ID number (e.g. T1004), the full request has been submitted and is either approved, disapproved or in process. If an entry has no ID number, then it represents a submission that has not yet been reviewed. Search terms are applied across an entire requests application including variables not displayed below. A more specific, detailed search may yield better results depending upon your needs.
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Investigator: Guojun Bu
Project Title: Biology and pathobiology of apoE in aging and Alzheimer’s disease (APOE U19 Core C: Neuropathology Core)
Date: February 16, 2022 at 3:54 pm
Request ID: T2206
Aim 1: Provide postmortem human brain tissue for biochemical and biophysical studies of apoE-lipoprotein particles (Core B) and for multi-omics studies (Core F) to define APOE-, aging-, and sex-dependent effects.
Aim 2: Perform ELISA assays to quantify levels of apoE, Aβ, tau, synaptic markers, glial activation markers, inflammatory cytokines, vascular markers, and other aging- and AD-related molecules in selected brain regions in AD and control brains.
Aim 3: Examine the association of APOE genotype with comorbid pathologies, including Aβ deposits and CAA, non-Alzheimer neuronal and glial tau pathologies, α-synuclein pathology and TDP-43 proteinopathy in AD and control brains.
Aim 4: Investigate neuropathologic phenotypes associated with novel genetic loci that modify APOE risk for AD identified in Project 5.
Investigator: Gilbert Gallardo
Project Title: Identifying the α2-Na/K ATPase singling pathways in reactive astrocyte for therapeutic intervention of Alzheimer’s disease
Date: February 14, 2022 at 11:16 pm
Request ID: T2203
Aim 1: To validate the upregulation of phospho-AMPK, phospho-mTOR and p62 and phospho-AKT in neuroinflammation in Human AD samples.
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Investigator: Brendan Lucey
Project Title: Characterization of Orexin/Hypocretin Kinetics in Alzheimer’s Disease
Date: February 14, 2022 at 5:33 pm
Request ID: T2204
Aim 1: In CSF samples previously collected by lumbar puncture from a cohort well-characterized for cognitive function, AD biomarkers, and sleep-wake activity, we will compare CSF prepro-orexin, orexin-A, and orexin-B in cognitively normal older adults without amyloid deposition.
Aim 2: In CSF samples previously collected by lumbar puncture from a cohort well-characterized for cognitive function, AD biomarkers, and sleep-wake activity, we will compare CSF prepro-orexin, orexin-A, and orexin-B in cognitively normal older adults with amyloid deposition.
Aim 3: In CSF samples previously collected by lumbar puncture from a cohort well-characterized for cognitive function, AD biomarkers, and sleep-wake activity, we will compare CSF prepro-orexin, orexin-A, and orexin-B in mildly impaired older adults
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Investigator: Gilbert Gallardo
Project Title: Determining the contribution of reactive astrocytes on amyloid-induced tauopathy
Date: February 14, 2022 at 5:26 pm
Request ID: T2205
Aim 1: The purpose of this tissue request is to investigate the contribution of reactive astrocytes on tau seeding and spreading in the presence of amyloid β plaques.
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Investigator: David Holtzman
Project Title: T cell surveillance and tau pathology in Alzheimer’s disease and primary tauopathies
Date: February 7, 2022 at 10:20 am
Request ID: T2202
Aim 1: Assess the relationship between T cell population and p-tau at different Braak stages of AD
Aim 2: Assess the relationship between T cell population and p-tau in primary tauopathies
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Investigator: Miles Berger
Project Title: APOE4 dependent effects on the Complement Pathway in human CSF from across the full adult lifespan
Date: January 24, 2022 at 10:12 am
Request ID: T2201
Aim 1: Determine the effect of APOE4 copy number on complement protein levels in the CSF in neurologically normal individuals across the adult life span (age 18-80)
Aim 2: Determine the effect of APOE4 copy number on complement pathway activation in the CSF in neurologically normal individuals across the adult life span (age 18-80)
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Investigator: Kido, Daniel
Project Title: Microhemorrrhages in the Elderly
Date: December 21, 2021 at 5:02 pm
Request ID: T9903
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Investigator: Finch, Caleb E. (Tuck)
Project Title: Characterization of complement factors and their association with A-beta deposits
Date: December 21, 2021 at 5:02 pm
Request ID: T9912
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Investigator: Smith, Mark A.
Project Title: Oxidative Damage via Mitochondria-derived Free Radical Accumulation
Date: December 21, 2021 at 5:02 pm
Request ID: T9908
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Investigator: Sun, Grace
Project Title: Immunohistochemistry identification of cPLA2 and COXII in control and AD brain
Date: December 21, 2021 at 5:02 pm
Request ID: T9901
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