The Knight ADRC has supported many investigators at Washington University and at other institutions over the years. We wish to avoid the situation where two investigators study the same research question to avoid duplication of effort and potential conflict. To determine if your topic has already been studied with our resources, please search our database. If you find that your topic or a related topic has been submitted, you may wish to contact the investigator to inquire about their findings to determine how you might proceed. You may wish to collaborate or modify your request to avoid overlap. The results below reflect requests made since online requests have been accepted. As such, not all fields will have data as certain information, such as aims, were not collected until recently. If an entry has been assigned an ID number (e.g. T1004), the full request has been submitted and is either approved, disapproved or in process. If an entry has no ID number, then it represents a submission that has not yet been reviewed. Search terms are applied across an entire requests application including variables not displayed below. A more specific, detailed search may yield better results depending upon your needs.
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Investigator: Rawan Tarawneh, MD
Project Title: CSF and Plasma Eotaxins As Diagnostic Biomarkers of Alzheimer Disease
Date: December 21, 2021 at 5:02 pm
Request ID: T2005
Aim 1: Examine the diagnostic value of CSF and plasma eotaxin-1 and eotaxin-3 in differentiating AD from controls
Aim 2: Investigate correlations of CSF and plasma eotaxins with CSF markers of amyloid and tau pathology (tau, p-tau181, Ab42) and markers of neuronal/synaptic injury (Ng and VILIP-1)
Aim 3: Examine associations of CSF and plasma eotaxins with cross-sectional and longitudinal cognitive assessments (including episodic memory, semantic memory, working memory, visuospatial, and global composite scores) and CDR-SB
Aim 4: Examine correlations of CSF and plasma eotaxins with whole brain and regional atrophy (cross-sectional only)
Investigator: Beau Ances
Project Title: Gut Dysbiosis and Intestional Dysfunction in AD
Date: December 21, 2021 at 5:02 pm
Request ID: T2006
Aim 1: Characterize gut bacterial content and organ permeability in adults in different stages of AD
Aim 2: Identify changes in bacterial content and organ permeability in individuals who advance to preclinical AD or clinical AD.
Aim 3: Compare bacterial content and organ permeability to AD biomarkers obtained from other projects.
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Investigator: Laura Ibanez
Project Title: Plasma cell-free RNA as non-invasive Biomarker for Neurodegeneration
Date: December 21, 2021 at 5:02 pm
Request ID: T2007
Aim 1: Develop a predictive model for Alzheimer Disease using RNA species free in Plasma
Aim 2: Replicate the predictive model in an independent sample of presymptomatic individuals
Aim 3: Test if the predictive model is agnostic to ethnic background
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Investigator: Russell Swerdlow
Project Title: CSF biomarker characterization in aging and AD
Date: December 21, 2021 at 5:02 pm
Request ID: T2008
Aim 1: Aim 1. Show feasibility to measure cerebrospinal fluid (CSF) A/T/N biomarkers in cognitively healthy older adults and individuals with AD.
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Investigator: Harari, Cruchaga and Karch
Project Title: A molecular and cellular atlas of Alzheimer�s Disease and Neuropath-free brains
Date: December 21, 2021 at 5:02 pm
Request ID: T2009
Aim 1: To identify glial- and neuronal-specific events that lead to AD by generating single-nuclei data for AD and neuropath-free brains
Aim 2: To perform cell-type specific differential expression analyses
Aim 3: To identify the cell-type gene co-expression and co-regulated networks disrupted by AD
Aim 4: To identify cell-type expression quantitative traits locus (Ct-eQTL) by integrating single-nuclei data with GWAS and whole genome/exome sequencing
Investigator: Marco Colonna
Project Title: MENINGEAL B CELLS IN AGING AND ALZHEIMER�S DISEASE
Date: December 21, 2021 at 5:02 pm
Request ID: T2010
Aim 1: TEST THE HYPOTHESIS THAT MENINGEAL B CELLS ORIGINATE FROM THE CALVARIAL BONE MARROW.
Aim 2: TO TEST THE HYPOTHESIS THAT THE DURA MENINGES B CELLS CLONALLY EXPAND DURING AGING AND BECOME AUTOREACTIVE ABC.
Aim 3: PROFILING THE MOLECULAR LANDSCAPE OF HUMAN DURA BY SINGLE-NUCLEI RNASEQ.
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Investigator: Chihiro Sato, Randall Bateman
Project Title: Quantitative analysis of tau phosphorylation and isoforms in Brain, CSF, and cell culture models
Date: December 21, 2021 at 5:02 pm
Request ID: T1722-C
Aim 1: The goal of this pilot experiment is to test the hypothesis that there are no brain regional differences in the phosphorylation profile of soluble tau in Alzheimer’s disease (AD) brain.
Aim 2: To test if there is difference in MTBR-tau in AD brain
Aim 3: To test if there is a correlation between local amyloid and p-tau in the brain
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Investigator: Benjamin Wolozin/Hu Li
Project Title: Interactions of Tau with RNA metabolism
Date: December 21, 2021 at 5:02 pm
Request ID: T2011
Aim 1: Compare signaling cascades induced by oligomeric tau and fibrillar tau
Aim 2: Determine how interaction of tau with m6A RNA varies with disease stage and type
Aim 3: Determine how m6A RNA transcripts vary with disease stage and type
Aim 4: Determine protein complexes associated with m6A RNA
Investigator: Gilbert Gallardo
Project Title: Targeting Reactive Astrocytes for Therapeutic Intervention of Alzheimer’s Disease
Date: December 21, 2021 at 5:02 pm
Request ID: T1920-A
Aim 1: The purpose of this tissue request if to address reviewers comments and evaluate the astrocytic �2-Na/K ATPase protein levels in Alzheimer’s disease patients.
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Investigator: Xiaoying Chen
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Date: December 21, 2021 at 5:02 pm
Request ID: T2012
Aim 1: To assess whether T-cell populations are elevated in AD brain compared to non-AD control
Aim 2: To characterize the functional interaction between T-cells and innate immune cells in AD and control brains
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