The Knight ADRC has supported many investigators at Washington University and at other institutions over the years. We wish to avoid the situation where two investigators study the same research question to avoid duplication of effort and potential conflict. To determine if your topic has already been studied with our resources, please search our database. If you find that your topic or a related topic has been submitted, you may wish to contact the investigator to inquire about their findings to determine how you might proceed. You may wish to collaborate or modify your request to avoid overlap. The results below reflect requests made since online requests have been accepted. As such, not all fields will have data as certain information, such as aims, were not collected until recently. If an entry has been assigned an ID number (e.g. T1004), the full request has been submitted and is either approved, disapproved or in process. If an entry has no ID number, then it represents a submission that has not yet been reviewed. Search terms are applied across an entire requests application including variables not displayed below. A more specific, detailed search may yield better results depending upon your needs.
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Investigator: P. De Jager, D. Bennett
Project Title: AMP AD diverse brain profiling project
Date: December 21, 2021 at 5:02 pm
Request ID: T2118
Aim 1: Generate bulk RNAseq profile from BA9, anterior caudate, and superior temporal gyrus
Aim 2: Generate Whole Genome Sequence Data frpm BA9
Aim 3: Generate TMT-based shotgun proteomic profile of BA9
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Investigator: Konrad Talbot
Project Title: Treating Alzheimer�s Disease by Reducing Brain Insulin Resistance with Incretin Receptor Agonists
Date: December 21, 2021 at 5:02 pm
Request ID: T2116
Aim 1: To quantify insulin responsiveness of the hippocampal formation (HF) from normal, MCI, and AD cases, test signaling responses of the HF from those cases to 1 and 10 nM insulin using an ex vivo insulin stimulation method validated for postmortem cases with PMIs less than 14 hours.
Aim 2: To test the ability of antidiabetics known as incretin receptor agonists (IRAs) to reduce known insulin resistance in the HF from MCI and AD cases by comparing the insulin responsiveness of the HF from such cases with or without IRA pretreatment using the ex vivo insulin stimulation method.
Aim 3: To test biochemical changes induced by IRAs that may account for their ability to reduce insulin resistance in the HF from MCI and AD cases based on preliminary data from ex vivo insulin stimulation experiments.
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Investigator: Wesley Horton
Project Title: Inflammatory Markers for Early Detection and Subtyping of Neurodegenerative and Mood Disorders Tissue Request to Washington University ADRC
Date: December 21, 2021 at 5:02 pm
Request ID: T2109
Aim 1: Determine the relationship of inflammatory markers in CSF and Plasma to clinical observations [Biomarker Signature Development]
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Investigator: Carlos Cruchaga
Project Title: Single cell and spatial transcriptomics of Alzheimer�s Disease brains
Date: December 21, 2021 at 5:02 pm
Request ID: T2110
Aim 1: Aim 1: To study the cell type-specific transcriptomic changes in AD brains
Aim 2: Aim 2: To perform spatial transcriptomic analyses.
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Investigator: Yuna Ayala
Project Title: Determining the mechanism of TDP-43 aggregate seeding and propagation
Date: December 21, 2021 at 5:02 pm
Request ID: T2112
Aim 1: Measure the seeding activity of human brain-derived pathological TDP-43 using different in vitro assays
Aim 2: Generate a model of TDP-43 aggregate seeding and propagation in vivo
Aim 3: Determine the role of TDP-43 aggregate seed origin and propagation pattern in neurodegeneration in a mouse model
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Investigator: Suzanne Schindler
Project Title: A blood test for symptomatic Alzheimer disease: Part 2
Date: December 21, 2021 at 5:02 pm
Request ID: T2111
Aim 1: Completion of fasting plasma Aβ42/Aβ40 dataset.
Aim 2: Comparison of plasma Aβ42/Aβ40 in fasted and non-fasted samples.
Aim 3: Expansion of dataset with non-fasted plasma with prioritization of samples from racial minorities.
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Investigator: Jason Ulrich/David Holtzman
Project Title: Effect of APOE genotype on localized transcriptomic response to pathology in AD
Date: December 21, 2021 at 5:02 pm
Request ID: T2113
Aim 1: Assess the effect of APOE isoform on localized transcriptomic profiles using spatial transcriptomis
Aim 2: Assess the effect of NFT and amyloid versus amyloid pathology on localized transcriptomic profiles
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Investigator: Chihiro Sato & Andrew Yoo
Project Title: Tau kinetics in neurons mimicking adult brain
Date: December 21, 2021 at 5:02 pm
Request ID: T2114
Aim 1: Profile tau, ptau and truncation in miNs with MAPT mutations
Aim 2: Tau SILK in miNs with MAPT mutations
Aim 3: Assessment of neurodegeneration and aggregated tau in miNs with MAPT mutations
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Investigator: Yabolonskiy, Dmitriy
Project Title: Quantifying Postmortem Neuronal Density for Correlation with qGRE MRI
Date: December 21, 2021 at 5:02 pm
Request ID: T2115
Aim 1: Pursue NeuN antigen retrieval for IHC of long-fixed FFPE brain specimens from qGRE MRI participants
Aim 2: Perform NeuN IHC on frozen brain tissue samples processed for IHC with short fixation protocol, digitally quantify neuron densities, correlate with qGRE MRI
Aim 3: Perform bulk-RNA-seq on frozen brain tissue samples from qGRE MRI participants, correlate neuronal complement with qGRE MRI
Aim 4: Perform sn-RNA-seq on frozen brain tissue samples from qGRE MRI participants, correlate neuronal complement with qGRE MRI
Investigator: Erin H. Norris
Project Title: Investigation of a prothrombotic state in AD patient plasma: Monitoring plasma contact system activation in AD pathophysiology
Date: December 21, 2021 at 5:02 pm
Request ID: T1306-B
Aim 1: Analysis of plasma contact system activation from large cohort of age- and sex-matched individuals diagnosed as non-demented (ND) or AD.
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