The Knight ADRC has supported many investigators at Washington University and at other institutions over the years. We wish to avoid the situation where two investigators study the same research question to avoid duplication of effort and potential conflict. To determine if your topic has already been studied with our resources, please search our database. If you find that your topic or a related topic has been submitted, you may wish to contact the investigator to inquire about their findings to determine how you might proceed. You may wish to collaborate or modify your request to avoid overlap. The results below reflect requests made since online requests have been accepted. As such, not all fields will have data as certain information, such as aims, were not collected until recently. If an entry has been assigned an ID number (e.g. T1004), the full request has been submitted and is either approved, disapproved or in process. If an entry has no ID number, then it represents a submission that has not yet been reviewed. Search terms are applied across an entire requests application including variables not displayed below. A more specific, detailed search may yield better results depending upon your needs.
Search Terms:
Investigator: David Holtzman
Project Title: Investigating the role of APOE in the progression of tau pathology in Alzheimer Disease
Date: December 21, 2021 at 5:02 pm
Request ID: T2013
Aim 1: Test whether astrocyte or microglia-derived APOE influences NP-tau seeding
Aim 2: Test whether the APOE Christchurch variant influences NP-tau seeding
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Investigator: Xiaoying Chen
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Date: December 21, 2021 at 5:02 pm
Request ID: T2012
Aim 1: To assess whether T-cell populations are elevated in AD brain compared to non-AD control
Aim 2: To characterize the functional interaction between T-cells and innate immune cells in AD and control brains
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Investigator: Gilbert Gallardo
Project Title: Targeting Reactive Astrocytes for Therapeutic Intervention of Alzheimer’s Disease
Date: December 21, 2021 at 5:02 pm
Request ID: T1920-A
Aim 1: The purpose of this tissue request if to address reviewers comments and evaluate the astrocytic �2-Na/K ATPase protein levels in Alzheimer’s disease patients.
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Investigator: Benjamin Wolozin/Hu Li
Project Title: Interactions of Tau with RNA metabolism
Date: December 21, 2021 at 5:02 pm
Request ID: T2011
Aim 1: Compare signaling cascades induced by oligomeric tau and fibrillar tau
Aim 2: Determine how interaction of tau with m6A RNA varies with disease stage and type
Aim 3: Determine how m6A RNA transcripts vary with disease stage and type
Aim 4: Determine protein complexes associated with m6A RNA
Investigator: Chihiro Sato, Randall Bateman
Project Title: Quantitative analysis of tau phosphorylation and isoforms in Brain, CSF, and cell culture models
Date: December 21, 2021 at 5:02 pm
Request ID: T1722-C
Aim 1: The goal of this pilot experiment is to test the hypothesis that there are no brain regional differences in the phosphorylation profile of soluble tau in Alzheimer’s disease (AD) brain.
Aim 2: To test if there is difference in MTBR-tau in AD brain
Aim 3: To test if there is a correlation between local amyloid and p-tau in the brain
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Investigator: Marco Colonna
Project Title: MENINGEAL B CELLS IN AGING AND ALZHEIMER�S DISEASE
Date: December 21, 2021 at 5:02 pm
Request ID: T2010
Aim 1: TEST THE HYPOTHESIS THAT MENINGEAL B CELLS ORIGINATE FROM THE CALVARIAL BONE MARROW.
Aim 2: TO TEST THE HYPOTHESIS THAT THE DURA MENINGES B CELLS CLONALLY EXPAND DURING AGING AND BECOME AUTOREACTIVE ABC.
Aim 3: PROFILING THE MOLECULAR LANDSCAPE OF HUMAN DURA BY SINGLE-NUCLEI RNASEQ.
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Investigator: Harari, Cruchaga and Karch
Project Title: A molecular and cellular atlas of Alzheimer�s Disease and Neuropath-free brains
Date: December 21, 2021 at 5:02 pm
Request ID: T2009
Aim 1: To identify glial- and neuronal-specific events that lead to AD by generating single-nuclei data for AD and neuropath-free brains
Aim 2: To perform cell-type specific differential expression analyses
Aim 3: To identify the cell-type gene co-expression and co-regulated networks disrupted by AD
Aim 4: To identify cell-type expression quantitative traits locus (Ct-eQTL) by integrating single-nuclei data with GWAS and whole genome/exome sequencing
Investigator: Erin H. Norris
Project Title: Investigation of a prothrombotic state in AD patient plasma: Monitoring plasma contact system activation in AD pathophysiology
Date: December 21, 2021 at 5:02 pm
Request ID: T1306-B
Aim 1: Analysis of plasma contact system activation from large cohort of age- and sex-matched individuals diagnosed as non-demented (ND) or AD.
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Investigator: Konrad Talbot
Project Title: Treating Alzheimer�s Disease by Reducing Brain Insulin Resistance with Incretin Receptor Agonists
Date: December 21, 2021 at 5:02 pm
Request ID: T2116
Aim 1: To quantify insulin responsiveness of the hippocampal formation (HF) from normal, MCI, and AD cases, test signaling responses of the HF from those cases to 1 and 10 nM insulin using an ex vivo insulin stimulation method validated for postmortem cases with PMIs less than 14 hours.
Aim 2: To test the ability of antidiabetics known as incretin receptor agonists (IRAs) to reduce known insulin resistance in the HF from MCI and AD cases by comparing the insulin responsiveness of the HF from such cases with or without IRA pretreatment using the ex vivo insulin stimulation method.
Aim 3: To test biochemical changes induced by IRAs that may account for their ability to reduce insulin resistance in the HF from MCI and AD cases based on preliminary data from ex vivo insulin stimulation experiments.
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Investigator: P. De Jager, D. Bennett
Project Title: AMP AD diverse brain profiling project
Date: December 21, 2021 at 5:02 pm
Request ID: T2118
Aim 1: Generate bulk RNAseq profile from BA9, anterior caudate, and superior temporal gyrus
Aim 2: Generate Whole Genome Sequence Data frpm BA9
Aim 3: Generate TMT-based shotgun proteomic profile of BA9
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