The Knight ADRC has supported many investigators at Washington University and at other institutions over the years. We wish to avoid the situation where two investigators study the same research question to avoid duplication of effort and potential conflict. To determine if your topic has already been studied with our resources, please search our database. If you find that your topic or a related topic has been submitted, you may wish to contact the investigator to inquire about their findings to determine how you might proceed. You may wish to collaborate or modify your request to avoid overlap. The results below reflect requests made since online requests have been accepted. As such, not all fields will have data as certain information, such as aims, were not collected until recently. If an entry has been assigned an ID number (e.g. T1004), the full request has been submitted and is either approved, disapproved or in process. If an entry has no ID number, then it represents a submission that has not yet been reviewed. Search terms are applied across an entire requests application including variables not displayed below. A more specific, detailed search may yield better results depending upon your needs.
Search Terms:
Investigator: Maria Catarina Silva
Project Title: Defining 4R-Tau Pathology in a New Cohort of IVS10+16 Neuronal Models for Therapeutic Development
Date: September 10, 2025 at 4:00 pm
Request ID: T2514
Aim 1: Model IVS10+16 tauopathy in iPSC-derived neurons.
Aim 2: Model IVS10+16 tauopathy using age-relevant neurons generated by direct transdifferentiation (MiN).
Aim 3: Dual neuronal cell models to support and evaluate novel small molecules therapeutics potential.
Aim 4:
Investigator: Andrew Yoo
Project Title: Modeling Neuronal Aging and Alzheimer’s Disease via 3D Direct Reprograming of Patient Fibroblast
Date: September 8, 2025 at 11:45 am
Request ID: T2508
Aim 1: Epigenetic basis for retrotransposon element dysregulation in neuronal aging
Aim 2: Effect of reducing RCAN1 function on neurodegeneration of directly reprogrammed AD neurons
Aim 3: Establishment of isogenic neuronal aging models through direct reprogramming
Aim 4:
Investigator: Miranda Orr
Project Title: Impact of Fixation and Post Mortem Interval Variables on Spatial Omics Data
Date: August 29, 2025 at 4:38 pm
Request ID: T2513
Aim 1: Test how ADRC fixation variables impact spatial proteomics data
Aim 2: Test how post mortem interval impact spatial proteomics data
Aim 3:
Aim 4:
Investigator: Elizabeth Pollina
Project Title: Activity-dependent genome plasticity in human aging and disease
Date: August 28, 2025 at 3:34 pm
Request ID: T2512
Aim 1: Characterize activity-dependent transcription and DNA damage repair in young, old and LOAD neurons.
Aim 2: Assess effects of NPAS4:NuA4 complex manipulation on ameliorating AD phenotypes.
Aim 3:
Aim 4:
Investigator: Justin Melendez
Project Title: Pseudotime Analysis of CSF Proteomics and Lipidomics for Alzheimer’s Disease Biomarker Discovery and Pathway Elucidation, Validated by Longitudinal Data
Date: August 15, 2025 at 11:10 am
Request ID: T2511
Aim 1: Develop and validate a pseudotime analysis of CSF proteomics to identify protein changes linked to amyloidosis progression and aging. Validate the model using longitudinal CSF data to pinpoint early biomarkers and drivers of amyloidosis.
Aim 2: Investigate the pseudotime trajectories of CSF proteomic changes in APOE4 carriers versus non-carriers to identify distinct aging and amyloidosis pathways. Validate these differences using longitudinal CSF data to identify specific biomarkers and pathways linked to APOE4-related Alzheimer’s risk.
Aim 3: Perform lipidomic profiling on longitudinal CSF samples using LC-MS to identify lipid biomarkers predictive of Alzheimer’s risk. Examine the influence of age, sex, APOE4 status, and Alzheimer’s pathology, and integrate proteomic and lipidomic data for comprehensive biomarker development.
Aim 4: Integrate lipidomic data with pseudotime analyses to explore combined proteomic and lipidomic changes over time. Use longitudinal CSF data to validate the integrated model, aiming to uncover comprehensive biomarkers and pathways that predict Alzheimer’s risk and progression.
Investigator: Takahisa Kanekiyo, David Holtzman, Alison Goate
Project Title: Determine apoE isoform-dependent changes incomposition of apoE lipoprotein particles in human CSF
Date: August 12, 2025 at 7:38 pm
Request ID: T2317-A
Aim 1: Purify apoE particles from human CSF samples usingantibody-based pull-down assay.
Aim 2: Assess the apoE particle size distribution with human CSFsamples.
Aim 3: Detect the apoE particles associated proteins and lipidsby proteomics and lipidomics profiling.
Aim 4:
Investigator: Marco Colonna
Project Title: Evaluating Vigil Neuroscience’s antibodies for their localization in brain tissue from patients with Alzheimer’s disease and cerebral amyloid angiopathy
Date: July 10, 2025 at 5:32 pm
Request ID: T2510
Aim 1: Vigil Neuroscience has developed antibodies targeting the TREM2 and its ligands. Through a sponsored research agreement, we aim to test their recognition of amyloid plaques in brain tissue from AD and CAA patients by immunohistochemistry.
Aim 2:
Aim 3:
Aim 4:
Investigator: Marijne Vandebergh
Project Title: Unravelling the genetic basis of disease penetrance and clinical heterogeneity in individuals with a pathogenic GRN mutation through omics-based approaches
Date: July 3, 2025 at 2:47 am
Request ID: T2509
Aim 1: To validate TMEM106B as modifier of disease penetrance
Aim 2: To identify novel genetic modifiers for onset age and clinical variability in individuals with FTD and GRN mutations
Aim 3:
Aim 4:
Investigator: Yuna Ayala
Project Title: Aggregation and TDP-43 dysfunction are tightly linked and amplified by impaired autoregulation and Ataxin-2
Date: July 2, 2025 at 1:43 pm
Request ID: T2112-A
Aim 1: Complete revision of a manuscript characterizing mechanisms that link TDP-43 aggregation to its mislocalization and loss of function, key features of ALS, FTD, and related dementias, and highlighting the potential of targeting this link for diagnosis and intervention.
Aim 2:
Aim 3:
Aim 4:
Investigator: Yuna Ayala
Project Title: Determining the mechanism of TDP-43 aggregate seeding and loss of function
Date: June 26, 2025 at 4:29 pm
Request ID: T2507
Aim 1: Aggregate seeding by insoluble FTD brain extract triggers progressive TDP-43 loss of function in cells. We aim to define factors that modulate this process and identify TDP-43 functions most sensitive to dysfunction. A submitted manuscript was favorably reviewed, tissue is critical for revision.
Aim 2: We will define how liquid-liquid phase separation controls TDP-43 pathology. ALS-linked mutations that reduce condensate fluidity enhance aggregation and seeding. We will test whether promoting liquid-like properties inhibits seeding and define how impaired phase separation drives pathology.
Aim 3: In collaboration with Drs. T. Miller and P. Kotzbauer (Washington University), we aim to develop novel TDP-43 aggregate inhibitors and degraders to reduce pathology and preserve function, using models of aggregation and dysfunction triggered by FTD-derived proteopathic seeds.
Aim 4: