The Knight ADRC has supported many investigators at Washington University and at other institutions over the years. We wish to avoid the situation where two investigators study the same research question to avoid duplication of effort and potential conflict. To determine if your topic has already been studied with our resources, please search our database. If you find that your topic or a related topic has been submitted, you may wish to contact the investigator to inquire about their findings to determine how you might proceed. You may wish to collaborate or modify your request to avoid overlap. The results below reflect requests made since online requests have been accepted. As such, not all fields will have data as certain information, such as aims, were not collected until recently. If an entry has been assigned an ID number (e.g. T1004), the full request has been submitted and is either approved, disapproved or in process. If an entry has no ID number, then it represents a submission that has not yet been reviewed. Search terms are applied across an entire requests application including variables not displayed below. A more specific, detailed search may yield better results depending upon your needs.
Search Terms:
Investigator: Jin-Moo Lee, Carlos Cruchaga and Manu Goyal
Project Title: Unraveling WMH Mechanisms in VCID with Imaging, Novel Multi-omics, and Open Data (UNWIND)
Date: [208]
Request ID: T2320 Goyal
Aim 1: Define cellular and molecular pathways involved in white matter disease and their relationship to CAA, arteriolosclerosis, and AD pathology, using single cell and spatial transcriptomics in post-mortem human brain tissue.
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Investigator: Richard Perrin
Project Title: AANP Diagnostic Slide Session
Date: [208]
Request ID: T2322
Aim 1: For submission as an unknown case in the AANP Diagnostic Slide Session (neuropathology educational session at national meeting)
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Investigator: Ryan Corces
Project Title: Identification of peripheral abnormalities in Alzheimer’s disease for biomarker and therapeutic discovery
Date: [208]
Request ID: T2401
Aim 1: Define the transcriptional changes that accompany the AD-specific immune signature in stimulated human monocytes and T cells using massive-scale multiplexed single-cell RNA-seq
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Investigator: David Holtzman
Project Title: Understanding the progression of tau pathology in Alzheimer Disease by genetic, environmental, and pharmacological factors
Date: [208]
Request ID: T2402
Aim 1: Determine how MSA4A function in microglia impacts NP-tau seeding and spreading
Aim 2: Understand whether depletion of gut microbiota using broad-spectrum antibiotics impacts NP-tau sedding and spreading
Aim 3: Determine if next-generation passive immunization therapies to clear amyloid plaques and therapeutic lowering of APOE using antisense oligonucleotides lowers NP-tau seeding and spreading
Aim 4: Determine if T cell depletion impacts NP tau seeding and spreading
Investigator: Sally Temple
Project Title: vascular and organoid modeling of neurodegenerative disease
Date: [208]
Request ID: T2403
Aim 1: examination of ELAVL4 immunostaining in cortical glutamatergic neurons in FTD-tau
Aim 2: Identify the RNA and protein interactome of ELAVL4 in FTD-tau
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Investigator: Iman beheshti
Project Title: Association between inflammation factors and brain aging in spectrum of AD
Date: [208]
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Aim 1: Using statistical and AI models to uncover the inflammation factors that faster brain aging in cognitively healthy, MCI and AD subjects with respect to sex.
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Investigator: Katherine Schwetye and Soumya Mukherjee
Project Title: Mass-spectroscopic characterization of N-terminal PTMs and isoform distribution of Aβ species in vascular deposits vs. parenchymal plaques
Date: [208]
Request ID: T2404
Aim 1: Determine the percentage of isomerization and isoform distribution at the N-terminus, and post-translational modifications of the amyloid-beta peptide in vessels using mass spectrometry (MS)
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Investigator: Nilufer Ertekin-Taner
Project Title: Centrally-linked Longitudinal pEripheral biomARkers of AD (CLEAR-AD) in multi-ethnic populations
Date: [208]
Request ID: T2209
Aim 1: Discover brain region and cell-specific molecular signatures of disease, and related endophenotypes, using single nucleus RNAseq (snRNAseq) and bulk tissue profiling of transcriptome, methylome, proteome, lipidome/metabolome data from 5 brain regions.
Aim 2: Identify molecular signatures preserved between brain and blood, using transcriptome, methylome, proteome, lipidome/metabolome data from antemortem blood collected on the same participants studied in Aim 1.
Aim 3: Discover molecular signatures that reflect biological subtypes and temporal progression of AD by performing integrative multi-omics analysis with endophenotypes including local neuropathology and antemortem longitudinal assessments.
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Investigator: Jonathan Kipnis
Project Title: Contribution of skull bone marrow-derived cells to Alzheimer’s disease
Date: [208]
Request ID: T2405
Aim 1: Assess a set of molecular markers, which we identified in engrafting myeloid cells in AD mouse model, in human tissues from AD and non-AD brains.
Aim 2: Correlate the expression of these molecular markers with AD pathology.
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Investigator: John Sninsky
Project Title: Development of the cell-free messenger-RNA based biomarkers for Alzheimer’s disease
Date: [208]
Request ID: T2406
Aim 1: Validate the performance of the cf-mRNA-based AD diagnostic classifier using an independent validation cohort.
Aim 2: Develop a predictive biomarker for MCI progression to AD (progressor vs non-progressor).
Aim 3: Evaluate the disease specificity of the cf-mRNA-based AD diagnostic classifier using plasma of other neurodegenerative diseases.
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